1CC8
CRYSTAL STRUCTURE OF THE ATX1 METALLOCHAPERONE PROTEIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 5ID-B |
| Synchrotron site | APS |
| Beamline | 5ID-B |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 1997-08-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.650, 29.600, 40.770 |
| Unit cell angles | 90.00, 114.83, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.020 |
| R-factor | 0.146 * |
| R-free | 0.17200 * |
| Structure solution method | DIRECT METHODS |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.050 |
| High resolution limit [Å] | 1.020 | 1.020 |
| Rmerge | 0.051 * | 0.106 * |
| Total number of observations | 102722 * | |
| Number of reflections | 24099 | |
| <I/σ(I)> | 8.4 | 5.3 |
| Completeness [%] | 95.4 | 90.2 |
| Redundancy | 4 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | or 18 degrees * |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | BENZAMIDINE | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | MES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.4 (M) | |
| 2 | 1 | reservoir | benzamidine hydrochloride | 2 (%) | |
| 3 | 1 | reservoir | glycerol | 3 (%) |
