1CBS

CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	45.650, 47.560, 77.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.23700
RMSD bond length	0.010
RMSD bond angle	1.510
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.059 *
Total number of observations	83399 *
Number of reflections	14678
Completeness [%]	90.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein-ligand complex		0.003ml
2	1	drop	Tris	20 (mM)
3	1	drop	monomethyl polyethylene glycol 5000	10 (%(v/v))
4	1	drop	sodium citrate	0.1 (M)
5	1	drop	dimethylsulphoxide	5 (%)
6	1	reservoir	monomethyl polyethylene glycol 5000	10 (%(v/v))
7	1	reservoir	sodium citrate	0.1 (M)
8	1	reservoir	dimethylsulphoxide	5 (%)