1CBI

APO-CELLULAR RETINOIC ACID BINDING PROTEIN I

Experimental procedure

Temperature [K]	294
Detector technology	AREA DETECTOR
Collection date	1993-11-16
Detector	SIEMENS
Spacegroup name	P 31 2 1
Unit cell lengths	127.450, 127.450, 53.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.000 - 2.700
R-factor	0.196
Rwork	0.196
R-free	0.23300
RMSD bond length	0.009
RMSD bond angle	27.300 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.000 *
High resolution limit [Å]	2.700 *	2.700 *
Rmerge	0.109	0.371 *
Number of reflections	12365
Completeness [%]	90.9	51 *
Redundancy	23	5.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-14 (mg/ml)
2	1	reservoir	PEG4000	16 (%)
3	1	reservoir		0.1 (mM)
4	1	reservoir	citrate	0.1 (mM)