1C9V
H12A VARIANT OF RIBONUCLEASE A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 275 |
| Detector technology | AREA DETECTOR |
| Collection date | 1998-04-02 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 64.540, 64.540, 65.080 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.700 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 17.919 * |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.030 | 0.130 |
| Total number of observations | 50643 * | |
| Number of reflections | 20142 | |
| <I/σ(I)> | 24 | |
| Completeness [%] | 96.0 | 88 |
| Redundancy | 2.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 20 * | 30% ammonium sulfate, 50% sodium chloride, 100mM sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 0.050 (M) | |
| 2 | 1 | drop | protein | 33 (mg/ml) | |
| 3 | 1 | drop | 25 (%(v/v)sat) | ||
| 4 | 1 | drop | ammonium sulfate | 15 (%(v/v)sat) | |
| 5 | 1 | reservoir | sodium acetate | 0.10 (M) | |
| 6 | 1 | reservoir | 50 (%(v/v)) | ||
| 7 | 1 | reservoir | ammonium sulfate | 30 (%(v/v)sat) |
