1C9O
CRYSTAL STRUCTURE ANALYSIS OF THE BACILLUS CALDOLYTICUS COLD SHOCK PROTEIN BC-CSP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-20 |
| Detector | MARRESEARCH |
| Spacegroup name | I 41 |
| Unit cell lengths | 76.995, 76.995, 47.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.170 |
| R-factor | 0.124 |
| Rwork | 0.124 |
| R-free | 0.17900 |
| Structure solution method | AB INITIO |
| RMSD bond length | 0.016 * |
| RMSD bond angle | 0.035 * |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 1.180 |
| High resolution limit [Å] | 1.170 | 1.170 |
| Rmerge | 0.041 | 0.301 |
| Number of reflections | 45174 | |
| <I/σ(I)> | 33.4 | |
| Completeness [%] | 96.2 | 38.8 |
| Redundancy | 4.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 293 | MPD, HEPES, pH 7.5. VAPOR DIFFUSION, HANGING DROP at 293 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.6 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) | |
| 4 | 1 | reservoir | MPD | 65 (%(v/v)) |
