1C9O

CRYSTAL STRUCTURE ANALYSIS OF THE BACILLUS CALDOLYTICUS COLD SHOCK PROTEIN BC-CSP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-20
Detector	MARRESEARCH
Spacegroup name	I 41
Unit cell lengths	76.995, 76.995, 47.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.170
R-factor	0.124
Rwork	0.124
R-free	0.17900
Structure solution method	AB INITIO
RMSD bond length	0.016 *
RMSD bond angle	0.035 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.180
High resolution limit [Å]	1.170	1.170
Rmerge	0.041	0.301
Number of reflections	45174
<I/σ(I)>	33.4
Completeness [%]	96.2	38.8
Redundancy	4.8	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	293	MPD, HEPES, pH 7.5. VAPOR DIFFUSION, HANGING DROP at 293

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10.6 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	HEPES	100 (mM)
4	1	reservoir	MPD	65 (%(v/v))