1C9L
PEPTIDE-IN-GROOVE INTERACTIONS LINK TARGET PROTEINS TO THE B-PROPELLER OF CLATHRIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.620, 130.787, 78.468 |
| Unit cell angles | 90.00, 115.47, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.22 * |
| Rwork | 0.221 |
| R-free | 0.26300 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.700 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.043 | 0.146 |
| Number of reflections | 27854 | |
| <I/σ(I)> | 19 | |
| Completeness [%] | 92.5 | 88.4 |
| Redundancy | 13.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 4 * | PEG 400, KOAc, DTT, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 15 (mM) | |
| 2 | 1 | drop | Tris-HCl | 1 (M) | |
| 3 | 1 | reservoir | PEG4000 | 21 (%) | |
| 4 | 1 | reservoir | potassium acetate | 350 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
