1C8Z
C-TERMINAL DOMAIN OF MOUSE BRAIN TUBBY PROTEIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1999-04-30 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.536, 51.023, 121.074 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.900 |
R-factor | 0.2102 |
Rwork | 0.210 |
R-free | 0.26500 |
RMSD bond length | 0.005 |
RMSD bond angle | 25.000 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.044 | 0.143 |
Total number of observations | 569516 * | |
Number of reflections | 21512 * | |
<I/σ(I)> | 30.26 | |
Completeness [%] | 98.0 | 93.3 |
Redundancy | 5 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 8 * | 293 | 2% PEG 4000, 0.1M HEPES, 4% 2-PROPANOL, 5MM DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP at 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris | 10 (mM) | |
3 | 1 | drop | 0.15 (M) | ||
4 | 1 | drop | dithiothreitol | 5 (mM) | |
5 | 1 | reservoir | PEG4000 | 2 (%) | |
6 | 1 | reservoir | HEPES | 0.1 (M) | |
7 | 1 | reservoir | 2-propanol | 4 (%) | |
8 | 1 | reservoir | dithiothreitol | 5 (mM) |