1C8X

Endo-Beta-N-Acetylglucosaminidase H, D130E Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-01-01
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	44.200, 55.200, 46.200
Unit cell angles	90.00, 103.80, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.197 *
Rwork	0.182
RMSD bond length	0.012
RMSD bond angle	25.420 *
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.080 *
Total number of observations	47618 *
Number of reflections	14192
Completeness [%]	96.1 *	92.7
Redundancy	3.09 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	293	30% PEG1000, 100 MM CACODYLATE, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293.K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein		0.002ml
2	1	drop	reservoir		0.002ml
3	1	reservoir	cacodylate	100 (mM)
4	1	reservoir	PEG1000	30 (%)