1C8W

THR45GLY VARIANT OF RIBONUCLEASE A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	275
Detector technology	AREA DETECTOR
Collection date	1995-10-08
Detector	SIEMENS HI-STAR
Spacegroup name	P 32 2 1
Unit cell lengths	64.320, 64.320, 64.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
R-factor	0.176 *
Rwork	0.176
RMSD bond length	0.011
RMSD bond angle	2.400 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.036	0.190
Total number of observations	32164 *
Number of reflections	18761
<I/σ(I)>	23.2
Completeness [%]	96.0	87
Redundancy	3.3	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	20 *	Ammonium sulfate, sodium chloride, sodium acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium acetate	0.050 (M)	pH5.6
2	1	drop	protein	60 (mg/ml)
3	1	drop	ammonium sulfate	15 (%(w/v))
4	1	drop		25 (%(w/v))
5	1	reservoir	sodium acetate	0.10 (M)	pH5.6
6	1	reservoir	ammonium sulfate	30 (%(w/v))
7	1	reservoir		50 (%(w/v))