1C8R
BACTERIORHODOPSIN D96N BR STATE AT 2.0 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.631, 60.631, 108.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 1.800 |
| R-factor | 0.126 |
| R-free | 0.18200 |
| Structure solution method | ISOMORPHOUS |
| Starting model (for MR) | 1c3w |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.057 | 0.216 |
| Total number of observations | 167219 * | |
| Number of reflections | 19005 | |
| <I/σ(I)> | 24 | 5.4 |
| Completeness [%] | 88.1 | 87.5 |
| Redundancy | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | other * | 5.6 | 20 * | Gabriele, R., (1998) J. Struct. Biol., 121, 82. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 3.3 (mg/ml) | |
| 2 | 1 | 1 | sodium potassium Pi | 1 (M) | |
| 3 | 1 | 1 | MPD | 2.5 (%) |
