1C8J
CRYSTAL STRUCTURE OF CYTOCHROME P450CAM MUTANT (F87W/Y96F)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.860, 61.990, 95.130 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.100 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.26400 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.719 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.085 | 0.431 |
| Number of reflections | 43708 | |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 95.6 | 98.1 |
| Redundancy | 2.7 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8000, Cacodylate, Sodium Acetate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
