1C8I

BINDING MODE OF HYDROXYLAMINE TO ARTHROMYCES RAMOSUS PEROXIDASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-08-15
Detector	FUJI
Spacegroup name	P 42 21 2
Unit cell lengths	74.400, 74.400, 117.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.000 - 2.000
R-factor	0.162
Rwork	0.162
R-free	0.22300
Structure solution method	OTHER
Starting model (for MR)	1gzb
RMSD bond length	0.018
RMSD bond angle	0.050
Data reduction software	PROCESS
Data scaling software	PROCESS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.250
High resolution limit [Å]	2.000	2.000
Rmerge	0.068	0.177
Total number of observations	108471 *
Number of reflections	21938
Completeness [%]	94.2	90.6
Redundancy	4.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	22-24 *	used seeding, Kunishima, N., (1993) Proteins: Struct., Funct., Genet., 15, 216. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ARP	20-40 (mg/ml)
2	1	reservoir	ammonium sulfate	32 (%sat)
3	1	reservoir	Tris-HCl	20 (mM)