1C8G

FELINE PANLEUKOPENIA VIRUS EMPTY CAPSID STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Collection date	1999-01-15
Detector	PRINCETON 2K
Spacegroup name	P 1 21 1
Unit cell lengths	266.170, 250.120, 379.100
Unit cell angles	90.00, 94.11, 90.00

Refinement procedure

Resolution	9.000 - 3.000
R-factor	0.245
RMSD bond length	0.010
RMSD bond angle	1.665
Data reduction software	DENZO
Phasing software	SnB
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.108	0.150
Number of reflections	358402
Completeness [%]	37.4	36.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.5 *	19 *	0.75-1.5% PEG8000, 8 mM CaCl2, 20 mM bis-Tris pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus	10 (mg/ml)
2	1	drop	Bis-Tris	20 (mM)
3	1	drop	Tris-HCl	20 (mM)
4	1	reservoir	PEG8000	0.75-1.50 (%)
5	1	reservoir		8 (mM)	or 5mM EDTA