1C81
MICHAELIS COMPLEX OF FRUCTOSE-2,6-BISPHOSPHATASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.100, 51.600, 49.300 |
| Unit cell angles | 90.00, 95.70, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.500 |
| Rwork | 0.209 |
| R-free | 0.28700 |
| RMSD bond length | 0.020 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 18.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.045 |
| Number of reflections | 6173 |
| <I/σ(I)> | 12.7 |
| Completeness [%] | 87.9 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.25 | 293 | PEG 4000, sodium chloride, Hepes, pH 7.25, EVAPORATION, temperature 293.0K |
