1C7Q
THE CRYSTAL STRUCTURE OF PHOSPHOGLUCOSE ISOMERASE/AUTOCRINE MOTILITY FACTOR/NEUROLEUKIN COMPLEXED WITH ITS CARBOHYDRATE PHOSPHATE INHIBITORS AND ITS SUBSTRATE RECOGNITION MECHANISM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-08-01 |
| Detector | RIGAKU |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 74.940, 93.640, 171.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.172 |
| Rwork | 0.172 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pgi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.650 | 2.440 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.075 | 0.316 |
| Total number of observations | 109736 * | |
| Number of reflections | 30710 | |
| <I/σ(I)> | 15 | 3.2 |
| Completeness [%] | 98.6 | 98 * |
| Redundancy | 3.57 | 3.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 0.1M HEPES PH 7.5, 0.8M K, NA TARTRATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | reservoir | potassium sodium tartrate | 0.8 (M) | |
| 4 | 1 | reservoir | HEPES | 0.1 (M) |
