1C6Q
T4 LYSOZYME MUTANT C54T/C97A IN THE PRESENCE OF 8 ATM KRYPTON
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 273 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-24 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 61.010, 61.010, 97.276 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.000 - 1.900 |
| Starting model (for MR) | 1l63 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 14.910 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.039 | 0.200 |
| Number of reflections | 16200 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 95.5 | 97.5 |
| Redundancy | 3.95 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 1.8-2.2 M NAH2/K2HPO4, PH 6.9-7.1, 50 MM BETA-MERCAPTOETHANOL AND/OR 50 MM HYDROXYETHYL DISULFIDE |
