1C6A

T4 LYSOZYME MUTANT C54T/C97A/L133A IN THE PRESENCE OF 8 ATM KRYPTON

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	1997-02-09
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 32 2 1
Unit cell lengths	61.113, 61.113, 97.444
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.000 - 2.100
Starting model (for MR)	1l69
RMSD bond length	0.020
RMSD bond angle	15.610 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.140
High resolution limit [Å]	2.100	2.100
Rmerge	0.064	0.247
Number of reflections	12780
<I/σ(I)>	9
Completeness [%]	99.6	99.2
Redundancy	3.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		4 *	1.8-2.2 M NAH2/K2HPO4, PH 6.9-7.1, 50 MM BETA-MERCAPTOETHANOL AND/OR 50 MM HYDROXYETHYL DISULFIDE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-20 (mg/ml)
2	1	reservoir	sodium/potassium phosphate	1.8-2.2 (M)
3	1	reservoir	beta-mercaptoethanol	50 (mM)	and/or hydroxyethyl disulfide