1C67

T4 LYSOZYME MUTANT C54T/C97A/L121A/L133A IN THE PRESENCE OF 8 ATM KRYPTON

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	273
Detector technology	IMAGE PLATE
Collection date	1999-03-11
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 32 2 1
Unit cell lengths	61.142, 61.142, 96.599
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.000 - 2.200
Starting model (for MR)	251l
RMSD bond length	0.020
RMSD bond angle	16.870 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.050	0.269
Number of reflections	11048
<I/σ(I)>	9.4
Completeness [%]	95.0 *	99.6
Redundancy	3.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *			1.8-2.2 M NAH2/K2HPO4, PH 6.9-7.1, 50 MM BETA-MERCAPTOETHANOL AND/OR 50 MM HYDROXYETHYL DISULFIDE