1C5E

BACTERIOPHAGE LAMBDA HEAD PROTEIN D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-04-01
Detector	MAR scanner 345 mm plate
Spacegroup name	P 1 21 1
Unit cell lengths	45.580, 69.070, 45.590
Unit cell angles	90.00, 104.32, 90.00

Refinement procedure

Resolution	10.000 - 1.100
R-factor	0.0982 *
R-free	0.13280
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	0.031
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXL-97
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.120
High resolution limit [Å]	1.100	1.100
Rmerge	0.046 *
Total number of observations	635194 *
Number of reflections	109996
Completeness [%]	99.2	98.3
Redundancy	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		28% PEG 4000, 0.1 M BIS-TRIS PH 6.5, 10 % GLYCEROL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris	20 (mM)
3	1	reservoir	PEG4000	28 (%)
4	1	reservoir	Bis-Tris	0.1 (M)
5	1	reservoir	glycerol	10 (%)