1C52
THERMUS THERMOPHILUS CYTOCHROME-C552: A NEW HIGHLY THERMOSTABLE CYTOCHROME-C STRUCTURE OBTAINED BY MAD PHASING
Experimental procedure
Source type | SYNCHROTRON |
Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
Synchrotron site | MPG/DESY, HAMBURG |
Beamline | BW6 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10 |
Detector | MARRESEARCH |
Spacegroup name | P 41 |
Unit cell lengths | 47.100, 47.100, 68.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 1.280 |
R-factor | 0.191 |
Rwork | 0.191 |
R-free | 0.22400 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 23.150 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MADSYS |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.600 | 1.310 |
High resolution limit [Å] | 1.280 | 1.280 |
Rmerge | 0.033 * | 0.357 * |
Total number of observations | 165576 * | |
Number of reflections | 36004 | |
<I/σ(I)> | 14.1 | 1.6 |
Completeness [%] | 93.6 | 78.2 |
Redundancy | 4.6 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 8.2 | drop consists of 8 microlitter protein and 2 microlitter reservoir solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris/HCl | 10 (mM) | |
3 | 1 | reservoir | Tris-HCl | 100 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 2.0 (M) |