1C50
IDENTIFICATION AND STRUCTURAL CHARACTERIZATION OF A NOVEL ALLOSTERIC BINDING SITE OF GLYCOGEN PHOSPHORYLASE B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-07-15 |
| Detector | MAR scanner 180 mm plate |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 129.174, 127.174, 116.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.24600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.110 | 0.445 |
| Total number of observations | 160475 * | |
| Number of reflections | 42617 | |
| <I/σ(I)> | 6.8 | 1.7 |
| Completeness [%] | 96.3 | 91 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | 16 * | Zographos, S.E., (1997) Structure (London), 5, 1413. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | W1807 | 1 (mM) | |
| 2 | 1 | 1 | enzyme | 27-28 (mg/ml) | |
| 3 | 1 | 1 | spermine | 1 (mM) | |
| 4 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 5 | 1 | 1 | BES | 10 (mM) | |
| 6 | 1 | 1 | EDTA | 0.1 (mM) | |
| 7 | 1 | 1 | sodium azide | 0.02 (%) |
