1C4P
BETA DOMAIN OF STREPTOKINASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-12-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.970, 83.990, 97.220 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.079 | 0.279 |
| Number of reflections | 23859 | |
| <I/σ(I)> | 17.4 | 3.1 |
| Completeness [%] | 98.1 | 90.3 |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.5 | 20 * | pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | PEG6000 | 25 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 100 (mM) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | MES | 100 (mM) |
