1C4K
ORNITHINE DECARBOXYLASE MUTANT (GLY121TYR)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 111.800, 111.800, 135.900 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.000 - 2.700 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ord MOLECULE A. |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.600 * |
| Data reduction software | DENZO (PACKAGE) |
| Data scaling software | SCALEPACK (PACKAGE) |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.069 * | 0.358 * |
| Number of reflections | 26013 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 94.3 | 95.1 |
| Redundancy | 2.8 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 * | 293 * | 20 DEG, SITTING DROP, MICROBRIDGES, RESERVOIRS CONTAINING 30% PEG3350, 0.2 M AMMONIUM ACETATE, AND 0.1 M SODIUM HEPES PH 7.0. DROPS CONSISTED OF EQUAL VOLUMES RESERVOIR AND 20 MG/ML PROTEIN SOLUTION. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium citrate-HCl | 100 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1.0 (mM) | |
| 4 | 1 | drop | PLP | 0.2 (mM) | |
| 5 | 1 | drop | sodium azide | 0.02 (%) | |
| 6 | 1 | drop | EDTA | 0.5 (mM) | |
| 7 | 1 | reservoir | PEG3350 | 30 (%) | |
| 8 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 9 | 1 | reservoir | Na HEPES | 100 (mM) |
