1C45
MUTANT HUMAN LYSOZYME WITH FOREIGN N-TERMINAL RESIDUES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 287 |
| Collection date | 1998-12-15 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.363, 61.480, 32.803 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.800 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.564 |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.038 |
| Total number of observations | 27473 * |
| Number of reflections | 9536 |
| Completeness [%] | 91.4 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.5 | pH 4.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 1.6-1.9 (M) | ||
| 2 | 1 | reservoir | acetate | 20 (mM) |
