1C3W
BACTERIORHODOPSIN/LIPID COMPLEX AT 1.55 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-07-01 |
| Detector | ADSC |
| Spacegroup name | P 63 |
| Unit cell lengths | 60.631, 60.631, 108.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 1.550 |
| R-factor | 0.158 |
| R-free | 0.22500 |
| Starting model (for MR) | 1brx |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.030 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 1.550 |
| Rmerge | 0.069 | 0.381 |
| Total number of observations | 372549 * | |
| Number of reflections | 34002 | |
| <I/σ(I)> | 19.9 | 2 |
| Completeness [%] | 99.1 | 97.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | CUBIC LIPID PHASE | 5.6 | 293 | Landau, E.M., (1996) Proc.Natl.Acad.Sci.USA., 93, 14532. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | sodium potassium Pi | 3.3 (M) | |
| 2 | 1 | 1 | protein | 3.5 (mg/ml) | |
| 3 | 1 | 1 | methylpentandiol | 0.05 (%) | |
| 4 | 1 | 1 | beta-octylglycopyranoside | 1.2 (%) |
