1C26

CRYSTAL STRUCTURE OF P53 TETRAMERIZATION DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1995-01-01
Detector	RIGAKU RAXIS IV
Spacegroup name	P 4 2 2
Unit cell lengths	45.450, 45.450, 33.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.700
R-factor	0.192
Rwork	0.192
RMSD bond length	0.013
RMSD bond angle	1.270
Phasing software	SQUASH
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.800
High resolution limit [Å]	1.700	1.700
Rmerge	0.037	0.187
Number of reflections	3950
<I/σ(I)>	20
Completeness [%]	98.9	0.903
Redundancy	5.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	298	3.0 M SODIUM FORMATE 0.5 M AMMONIUM SULFATE 50 MM TRIS-HCL PH 8.8, VAPOR DIFFUSION, HANGING DROP at 298 K