1C1H
CRYSTAL STRUCTURE OF BACILLUS SUBTILIS FERROCHELATASE IN COMPLEX WITH N-METHYL MESOPORPHYRIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | MAX II BEAMLINE I711 |
Synchrotron site | MAX II |
Beamline | I711 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-03-19 |
Detector | MAR scanner 345 mm plate |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 49.970, 58.680, 98.050 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 1.900 |
R-factor | 0.181 |
Rwork | 0.181 |
R-free | 0.23100 |
RMSD bond length | 0.005 |
RMSD bond angle | 22.000 * |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.079 | 0.342 |
Number of reflections | 21475 | |
<I/σ(I)> | 13.8 | |
Completeness [%] | 91.8 | 91.6 |
Redundancy | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 2000, MAGNESIUM CHLORIDE, TRIS-HCL, pH 8.5, VAPOR DIFFUSION, SITTING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 1 (mM) | |
2 | 1 | drop | N-MeMP | 1 (,<) | |
3 | 1 | reservoir | PEG2000 | 30 (%(w/v)) | |
4 | 1 | reservoir | 0.2 (M) | ||
5 | 1 | reservoir | Tris-HCl | 100 (mM) | |
6 | 1 | reservoir | 0.5 (mM) |