1C10

CRYSTAL STRUCTURE OF HEW LYSOZYME UNDER PRESSURE OF XENON (8 BAR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-02-08
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	78.530, 78.530, 37.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.900 - 2.030
R-factor	0.17
Structure solution method	OTHER
Starting model (for MR)	1lse
RMSD bond length	0.006
RMSD bond angle	0.023
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	9.900	2.150
High resolution limit [Å]	2.030	2.030
Rmerge	0.044	0.152
Number of reflections	6454
<I/σ(I)>	8.9	4.3
Completeness [%]	79.0	59
Redundancy	5.1	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5 *	20 *	SODIUM CHLORIDE, PH = 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	elastase	2 (%)
2	1	1	sodium acetate	0.01 (M)
3	1	1	sodium sulfate	0.02 (M)