1C0W
CRYSTAL STRUCTURE OF THE COBALT-ACTIVATED DIPHTHERIA TOXIN REPRESSOR-DNA COMPLEX REVEALS A METAL BINDING SH-LIKE DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08-15 |
| Detector | CUSTOM-MADE |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 180.200, 180.200, 151.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.200 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1DTR |
| RMSD bond length | 0.130 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 3.200 | |
| Rmerge | 0.120 * | 0.450 * |
| Total number of observations | 772772 * | |
| Completeness [%] | 100.0 * | 99.9 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | Tris | 10 (mM) | |
| 3 | 1 | drop | dithiothreitol | 10 (mM) | |
| 4 | 1 | drop | oligonucleotide solution | 0.155 (mM) | |
| 5 | 1 | reservoir | MES | 100 (mM) | |
| 6 | 1 | reservoir | polyvinylalcohol15000 | 7.5 (%) | |
| 7 | 1 | reservoir | 5 (mM) |
