1C08

CRYSTAL STRUCTURE OF HYHEL-10 FV-HEN LYSOZYME COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	MACSCIENCE
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-09-15
Detector	MACSCIENCE
Spacegroup name	P 41 21 2
Unit cell lengths	57.208, 57.208, 236.071
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.300
Rwork	0.235
R-free	0.23500 *
RMSD bond length	0.016
RMSD bond angle	2.500
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000	2.400
High resolution limit [Å]	2.300	2.300
Rmerge	0.076	0.265
Number of reflections	18301
<I/σ(I)>	11
Completeness [%]	98.5	97.6
Redundancy	8	8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	20 *	HEPES, PEG6000, MPD, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	PEG6000	9-11 (%(w/v))
3	1	reservoir	MPD	7-9 (%(w/v))