1C03
CRYSTAL STRUCTURE OF YPD1P (TRICLINIC FORM)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-11-20 |
| Detector | FUJI |
| Spacegroup name | P 1 |
| Unit cell lengths | 66.343, 66.480, 66.491 |
| Unit cell angles | 106.37, 106.66, 115.33 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.206 |
| Rwork | 0.206 |
| R-free | 0.24800 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.940 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.044 | 0.260 |
| Number of reflections | 35254 * | |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 88.3 | 85.9 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.36 | 296 * | PEG 400, ammonium sulfate, Hepes, Li2SO4, pH 7.36, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 23 (mg/ml) |
