1BZP
ATOMIC RESOLUTION CRYSTAL STRUCTURE ANALYSIS OF NATIVE DEOXY AND CO MYOGLOBIN FROM SPERM WHALE AT ROOM TEMPERATURE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04-01 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.150, 31.040, 64.590 |
| Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
| Resolution | 12.000 - 1.150 |
| R-factor | 0.114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mbn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.031 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.200 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.062 | 0.310 |
| Total number of observations | 175253 * | |
| Number of reflections | 41207 | |
| <I/σ(I)> | 19.9 | 2.7 |
| Completeness [%] | 85.9 | 66.1 |
| Redundancy | 4.2 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.9 | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULPHATE, 0.1 M POTASSIUM PHOSPHATE., pH 5.9 |
