1BZ7

FAB FRAGMENT FROM MURINE ASCITES

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	300
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	146.800, 56.000, 80.000
Unit cell angles	90.00, 119.20, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.162
Rwork	0.162
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MERLOT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000
High resolution limit [Å]	2.500
Rmerge	0.050 *
Total number of observations	79380 *
Number of reflections	18143
Completeness [%]	94.0
Redundancy	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	zinc acetate	0.2 (M)
2	1	reservoir	sodium cacodylate	0.1 (M)
3	1	reservoir	PEG8000	23 (%)