1BYS

CRYSTAL STRUCTURE OF NUC COMPLEXED WITH TUNGSTATE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	93
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 43 21 2
Unit cell lengths	59.250, 59.250, 111.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.203
Rwork	0.203
R-free	0.27200
RMSD bond length	0.015
RMSD bond angle	25.810 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.800	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.069 *	0.150 *
Total number of observations	119802 *
Number of reflections	17248
<I/σ(I)>	20	20
Completeness [%]	90.0	99
Redundancy	6.94	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		Zhao, Y., (1997) Protein Sci., 6, 2655. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	ammonium sulfate	1 (M)
3	1	drop	Tris-HCl	50 (mM)