1BYO

WILD-TYPE PLASTOCYANIN FROM SILENE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	285
Detector technology	IMAGE PLATE
Collection date	1994-12-15
Detector	RIGAKU
Spacegroup name	P 32 2 1
Unit cell lengths	76.600, 76.600, 65.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.189
Rwork	0.197
R-free	0.22500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PCY
RMSD bond length	0.012
RMSD bond angle	0.016
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.000
Rmerge	0.060
Total number of observations	44196 *
Number of reflections	11515
Completeness [%]	94.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7 *		53% SATURATED AMMONIUM SULFATE, 0.1 M SODIUM ACETATE BUFFER, pH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.8 (M)
2	1	reservoir	potassium phosphate	100 (mM)
3	1	drop	protein	13 (mg/ml)