1BY9
CRYSTAL STRUCTURE OF THE E2 DNA-BINDING DOMAIN FROM HUMAN PAPILLOMAVIRUS TYPE-16: IMPLICATIONS FOR ITS DNA BINDING-SITE SELECTION MECHANISM
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.775, 43.775, 76.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 99.000 - 2.200 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bop |
| RMSD bond length | 0.006 |
| RMSD bond angle | 23.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | |
| High resolution limit [Å] | 2.200 | 2.200 * |
| Rmerge | 0.118 * | 0.314 * |
| Total number of observations | 32566 * | |
| Number of reflections | 4165 | |
| Completeness [%] | 98.8 * | 99.8 * |
| Redundancy | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.2 | pH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1 (mg/ml) | |
| 10 | 1 | reservoir | glycerol | 10 (%(v/v)) | |
| 11 | 1 | reservoir | imidazole | 50 (mM) | pH6.8 |
| 2 | 1 | drop | MES | 6 (mM) | pH6.2 |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | 0.3 (mM) | ||
| 6 | 1 | drop | ammonium sulfate | 0.9 (M) | |
| 7 | 1 | drop | glycerol | 5 (%(v/v)) | |
| 8 | 1 | drop | imidazole | 25 (mM) | pH6.8 |
| 9 | 1 | reservoir | ammonium sulfate | 1.8-2 (M) |
