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STRUCTURE OF M2BP SCAVENGER RECEPTOR CYSTEINE-RICH DOMAIN

Experimental procedure

Source type	ROTATING ANODE
Source details	ELLIOTT GX-21
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-07-07
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	44.630, 44.630, 134.540
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.186
Rwork	0.186
R-free	0.21800
Structure solution method	MIRAS
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	SCALA ((CCP4))
Phasing software	CCP4
Refinement software	X-PLOR (3.84)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.110
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.186
Total number of observations	60557 *
Number of reflections	9843
<I/σ(I)>	7.3	3.7
Completeness [%]	99.6	100
Redundancy	6.2	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	Tris	10 (mM)
3	1	reservoir	Bis-Tris	50 (mM)
4	1	reservoir	PEG1500	30-32 (%)