1BX0

Ferredoxin:nadp+ oxidoreductase (ferredoxin reductase) mutant e312l

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SDMS
Spacegroup name	C 1 2 1
Unit cell lengths	91.200, 58.000, 68.200
Unit cell angles	90.00, 99.90, 90.00

Refinement procedure

Resolution	10.000 - 1.900
R-factor	0.19
Rwork	0.190
Structure solution method	OTHER
Starting model (for MR)	1fnb
RMSD bond length	0.012
RMSD bond angle	24.900 *
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.110 *	0.375 *
Total number of observations	115494 *
Number of reflections	27690
<I/σ(I)>	13.8	2.5
Completeness [%]	99.7	97.8
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	4 *	drop is produced by a 1:1(v/v) mixture of the stored FNR stock with a reservoir *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	1.5 (M)
2	1	reservoir	sodium citrate/phosphate	0.2 (M)	McIlvaine's buffer