1BWV
Activated Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase (RUBISCO) Complexed with the Reaction Intermediate Analogue 2-Carboxyarabinitol 1,5-Bisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03-15 |
| Detector | FUJI |
| Spacegroup name | P 64 |
| Unit cell lengths | 117.070, 117.070, 319.630 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| R-free | 0.19700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rbl |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.550 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.097 | 0.203 |
| Total number of observations | 277331 * | |
| Number of reflections | 83303 * | |
| Completeness [%] | 86.4 | 58.1 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 293 * | 9% PEG8000, 4% MPD, 50 MM HEPES PH7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.0 (M) | |
| 2 | 1 | drop | 20 (mM) | ||
| 3 | 1 | drop | 20 (mM) | ||
| 4 | 1 | drop | 2-CABP | 2 (mM) | |
| 5 | 1 | reservoir | HEPES | 50 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 9 (%) | |
| 7 | 1 | reservoir | MPD | 4 (%) |
