1BWU
MANNOSE-SPECIFIC AGGLUTININ (LECTIN) FROM GARLIC (ALLIUM SATIVUM) BULBS COMPLEXED WITH ALPHA-D-MANNOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.243, 43.780, 79.268 |
| Unit cell angles | 90.00, 112.37, 90.00 |
Refinement procedure
| Resolution | 9.000 - 2.800 |
| R-factor | 0.226 |
| Rwork | 0.226 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SNOWDROP LECTIN (PDB ENTRY 1MSA) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.600 |
| Data reduction software | AUTOMAR |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 9.000 | 2.800 | 2.550 |
| High resolution limit [Å] | 2.400 | 2.550 | 2.400 |
| Rmerge | 0.059 | 0.226 | 0.369 |
| Number of reflections | 22244 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 86.6 | 12.2 | |
| Redundancy | 2.31 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 * | Chandra, N.R., (1997) Acta Crystallogr., Sect.D, 53, 787. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.5 (mg/ml) | |
| 2 | 1 | drop | phosphate-buffered saline | 20 (mM) | |
| 3 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 4 | 1 | reservoir | phosphate-buffered saline | 20 (mM) |
