1BWS

CRYSTAL STRUCTURE OF GDP-4-KETO-6-DEOXY-D-MANNOSE EPIMERASE/REDUCTASE FROM ESCHERICHIA COLI A KEY ENZYME IN THE BIOSYNTHESIS OF GDP-L-FUCOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1997-08-15
Detector	RIGAKU RAXIS
Spacegroup name	P 32 2 1
Unit cell lengths	104.200, 104.200, 75.880
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.202 *
R-free	0.28700 *
Structure solution method	MIR
RMSD bond length	0.016
RMSD bond angle	1.650
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.200
Rmerge	0.057
Total number of observations	105130 *
Number of reflections	24481
<I/σ(I)>	13.6
Completeness [%]	99.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	20 *	pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	lithium sulphate	1.5 (M)
2	1	reservoir	MES	0.1 (M)
3	1	drop	protein	16 (mg/ml)