1BW8
MU2 ADAPTIN SUBUNIT (AP50) OF AP2 ADAPTOR (SECOND DOMAIN), COMPLEXED WITH EGFR INTERNALIZATION PEPTIDE FYRALM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 64 |
| Unit cell lengths | 125.680, 125.680, 73.160 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.000 - 2.650 |
| R-factor | 0.272 |
| Rwork | 0.250 |
| R-free | 0.29600 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.038 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.790 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Rmerge | 0.089 * | 0.882 |
| Number of reflections | 119506 | |
| <I/σ(I)> | 21.3 | 2.1 |
| Completeness [%] | 99.0 | 96.7 * |
| Redundancy | 9.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 16 * | HANGING DROP, 2.2M NACL, 0.4M NA/K PHOSPHATE, 10MM DTT 0.1M MES PH 7.1, 15% GLYCEROL, 16DEGREES, MOLAR RATIO OF PEPTIDE TO PROTEIN 3:1, vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2.2 (M) | ||
| 2 | 1 | reservoir | sodium potassium phosphate | 0.4 (M) | |
| 3 | 1 | reservoir | dithiothreitol | 10 (mM) | |
| 4 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 5 | 1 | reservoir | MES | 0.1 (M) |
