1BVX
THE 1.8 A STRUCTURE OF GEL GROWN TETRAGONAL HEN EGG WHITE LYSOZYME
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.203, 79.203, 38.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 193l |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.190 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | |
| High resolution limit [Å] | 1.700 | 1.700 * |
| Rmerge | 0.068 | 0.232 * |
| Total number of observations | 68445 * | |
| Number of reflections | 12433 | |
| <I/σ(I)> | 21.6 | |
| Completeness [%] | 89.6 | 51.8 * |
| Redundancy | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | liquid-gel diffusion * | 4.5 | HEWL CRYSTALS WERE GROWN IN A GELLED PROTEIN SOLUTION WITH AGAROSE (SIGMA, TYPE VII: OF LOW GELLING TEMPERATURE), 4 % MPD IN ACETATE BUFFER (PH 4.5), THE PRECIPITATING AGENT SOL MPD IN BUFFER. THE GLASS TUBE USED WAS 6 CM TALL WITH AN IN CRYSTALS OF TYPICAL DIMENSIONS $SIM$0.7 $TIMES$ 0.6 $TIM, grown in gelled protein solution with agarose |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 25 (mg/ml) | |
| 2 | 1 | 1 | agarose | 0.2 (%) | |
| 3 | 1 | 1 | 4 (%) | 0.68M | |
| 4 | 1 | 1 | MPD | 4 (%) | |
| 5 | 1 | 2 | 15 (%) | 2.57M | |
| 6 | 1 | 2 | MPD | 15 (%) |
