1BVX

THE 1.8 A STRUCTURE OF GEL GROWN TETRAGONAL HEN EGG WHITE LYSOZYME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	79.203, 79.203, 38.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.182
Rwork	0.182
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	193l
RMSD bond length	0.008
RMSD bond angle	22.190 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	99.000
High resolution limit [Å]	1.700	1.700 *
Rmerge	0.068	0.232 *
Total number of observations	68445 *
Number of reflections	12433
<I/σ(I)>	21.6
Completeness [%]	89.6	51.8 *
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	liquid-gel diffusion *	4.5		HEWL CRYSTALS WERE GROWN IN A GELLED PROTEIN SOLUTION WITH AGAROSE (SIGMA, TYPE VII: OF LOW GELLING TEMPERATURE), 4 % MPD IN ACETATE BUFFER (PH 4.5), THE PRECIPITATING AGENT SOL MPD IN BUFFER. THE GLASS TUBE USED WAS 6 CM TALL WITH AN IN CRYSTALS OF TYPICAL DIMENSIONS $SIM$0.7 $TIMES$ 0.6 $TIM, grown in gelled protein solution with agarose

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	25 (mg/ml)
2	1	1	agarose	0.2 (%)
3	1	1		4 (%)	0.68M
4	1	1	MPD	4 (%)
5	1	2		15 (%)	2.57M
6	1	2	MPD	15 (%)