1BVK

HUMANIZED ANTI-LYSOZYME FV COMPLEXED WITH LYSOZYME

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1996-11
Detector	RIGAKU
Spacegroup name	P 41 21 2
Unit cell lengths	97.700, 97.700, 174.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.700
R-factor	0.208
Rwork	0.208
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HULYS STRUCTURE AND LYSOZYME FROM PDB ENTRY 1VFB
RMSD bond length	0.014
RMSD bond angle	27.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.075	0.452
Total number of observations	120820 *
Number of reflections	22379
<I/σ(I)>	9.6
Completeness [%]	93.2	73.5
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5		pH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	7-10 (mg/ml)
2	1	reservoir	phosphate	1.6-1.7 (M)
3	1	reservoir	HEPES	0.1 (M)