1BUU
ONE HO3+ FORM OF RAT MANNOSE-BINDING PROTEIN A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1994-05-15 |
Detector | RIGAKU |
Spacegroup name | P 21 3 |
Unit cell lengths | 94.400, 94.400, 94.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.21400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1rtm CHAIN 1 |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | CNS (0.3C) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.056 | 0.215 |
Number of reflections | 20530 | |
Completeness [%] | 95.1 | 88 |
Redundancy | 7 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | HANGING-DROP VAPOR DIFFUSION AGAINST RESERVOIR CONTAINING 11-15% (W/V) PEG 3350, 0.325 MM HOCL3, 100 MM TRIS-CL PH 8.0, 10 MM NACL, 0.02% NAN3., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 20 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 8-12 (%(w/v)) | |
3 | 1 | reservoir | Tris-Cl | 100 (mM) | pH8.0 |
4 | 1 | reservoir | 10 (mM) | ||
5 | 1 | reservoir | 0.02 (%) | ||
6 | 1 | reservoir | 0.375 (mM) |