1BUP

T13S MUTANT OF BOVINE 70 KILODALTON HEAT SHOCK PROTEIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-01-01
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	143.572, 64.164, 46.236
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	100.000 - 1.700
R-factor	0.189
Rwork	0.189
R-free	0.22000
Structure solution method	OTHER
Starting model (for MR)	1hpm
RMSD bond length	0.011
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	1.740
High resolution limit [Å]	1.700	1.700
Rmerge	0.044 *	0.142 *
Total number of observations	143963 *
Number of reflections	46104
Completeness [%]	97.4	90.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	9	4 *	pH 9.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MgATP	1 (mM)
2	1	reservoir	CHES	50 (mM)
3	1	reservoir		1 (M)
4	1	reservoir	PEG8000	20 (%)