1BUO
BTB DOMAIN FROM PLZF
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Wavelength(s) | 0.9638, 0.9790, 0.9793 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 72.630, 72.630, 168.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.25200 |
| Structure solution method | MAD |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.042 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.044 * | 0.248 * |
| Total number of observations | 204897 * | |
| Number of reflections | 18253 | |
| <I/σ(I)> | 11.2 | 3 |
| Completeness [%] | 99.3 | 96.7 |
| Redundancy | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 4 * | 0.15 M CACL2, 0.1 M HEPES (PH 7.5), 4% ISOPROPANOL, |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | 300 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 50 (mM) | |
| 4 | 1 | drop | TCEP | 1 (mM) | |
| 5 | 1 | reservoir | 0.15 (M) | ||
| 6 | 1 | reservoir | HEPES | 0.1 (M) | |
| 7 | 1 | reservoir | isopropanol | 4 (%) |
