1BUO

BTB DOMAIN FROM PLZF

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	0.9638, 0.9790, 0.9793
Spacegroup name	I 41 2 2
Unit cell lengths	72.630, 72.630, 168.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.212 *
Rwork	0.212
R-free	0.25200
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	0.042
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	SHARP
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.044 *	0.248 *
Total number of observations	204897 *
Number of reflections	18253
<I/σ(I)>	11.2	3
Completeness [%]	99.3	96.7
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	0.15 M CACL2, 0.1 M HEPES (PH 7.5), 4% ISOPROPANOL,

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop		300 (mM)
3	1	drop	Tris-HCl	50 (mM)
4	1	drop	TCEP	1 (mM)
5	1	reservoir		0.15 (M)
6	1	reservoir	HEPES	0.1 (M)
7	1	reservoir	isopropanol	4 (%)