1BUN
STRUCTURE OF BETA2-BUNGAROTOXIN: POTASSIUM CHANNEL BINDING BY KUNITZ MODULES AND TARGETED PHOSPHOLIPASE ACTION
Experimental procedure
| Temperature [K] | 293 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992-06 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 52.600, 52.600, 177.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.450 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| R-free | 0.28100 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.600 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.450 |
| Rmerge | 0.069 |
| Total number of observations | 30515 * |
| Number of reflections | 9372 |
| Completeness [%] | 96.4 |
| Redundancy | 3.25 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | 20 * | pH 8.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | EDTA | 0.5 (mM) | |
| 3 | 1 | drop | 0.01 (%) | ||
| 4 | 1 | drop | 1.4 (M) | ||
| 5 | 1 | reservoir | 3.3 (M) | ||
| 6 | 1 | reservoir | Tris-HCl | 50 (mM) |
