1BUA
STRUCTURAL AND ENERGETIC ORIGINS OF INDIRECT READOUT IN SITE-SPECIFIC DNA CLEAVAGE BY A RESTRICTION ENDONUCLEASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | RIGAKU |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.300, 64.200, 49.400 |
| Unit cell angles | 109.30, 108.30, 96.30 |
Refinement procedure
| Resolution | 6.000 - 2.150 |
| R-factor | 0.2 * |
| Rwork | 0.200 |
| R-free | 0.28800 |
| Structure solution method | OTHER |
| Starting model (for MR) | PDB 1AZ0 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.940 |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.900 | |
| Rmerge | 0.056 | |
| Total number of observations | 83222 * | |
| Number of reflections | 37374 | |
| Completeness [%] | 86.0 | 75.4 * |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | pH 7.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10.2 (mg/ml) | |
| 2 | 1 | drop | DNA | 1.8 (mg/ml) | |
| 3 | 1 | reservoir | PEG4000 | 5-10 (%) | |
| 4 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
| 5 | 1 | reservoir | 150 (mM) | ||
| 6 | 1 | reservoir | 5'-AAAGACITCTT | 25 (mM) |
