1BU8
RAT PANCREATIC LIPASE RELATED PROTEIN 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.393, 79.133, 60.940 |
| Unit cell angles | 90.00, 102.07, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.24400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1thg |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.420 |
| Number of reflections | 47899 | |
| <I/σ(I)> | 9.1 | 2.3 |
| Completeness [%] | 97.5 | 81.9 |
| Redundancy | 2.4 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 18 (mg/ml) | |
| 2 | 1 | drop | 0.2 (M) | ||
| 3 | 1 | drop | Tris-HCl | 0.1 (M) | |
| 4 | 1 | reservoir | PEG8000 | 8 (%) | |
| 5 | 1 | reservoir | Tris-HCl | 0.1 (M) |
